Nitrogen and sulfur co-doped carbon quantum dots fluorescence quenching assay for detection of mercury (II)

Mercury is a highly toxic and potentially bioaccumulative heavy metal ion that can cause severe health problems in humans even at very low concentrations. Thus, the development of a simple, rapid, and sensitive assay for the effective detection of mercury ions at trace levels is of great importance. Here, nitrogen and sulfur co-doped carbon quantum dots (N,S-CQD) were synthesized by a simple hydrothermal treatment of chitosan in the presence of thiourea and citric acid with a quantum yield (QY) up to 33.0 % and used as a selective fluorescent probe to detect mercury ions (Hg²⁺). The effect of pH, ionic strength, and time on the fluorescence intensity of N,S-CQD were investigated and optimized.

The synthesized N,S-CQD showed ultrasensitive ability to detect Hg²⁺ ions in the water samples, also in the presence of 11 interfering metal ions, with a low detection limit (∼4 nM) over a wide linear range from ∼5–160 nM. The sensing performance of N,S-CQD probe in real sample applications was evaluated by the detection of Hg²⁺ in lake water samples, which confirmed its potential application in environmental analysis.